Genetic evidence points to critical role of skate nursery areas, and a possible new species
by NOAA Fisheries 16 Aug 2020 09:17 UTC
A skate egg case, or `mermaid's purse.` © NOAA Fisheries
Skates are an important predator and widely distributed across Alaska marine ecosystems. There is interest in developing commercial fisheries for them.
However, skates are vulnerable to fishing impacts because they typically develop slowly and do not reproduce until later in life. Science can provide a basis for ensuring sustainable fishery development and helping to protect critical life stages and habitat.
NOAA Fisheries scientists conducted an important genetic study using next-generation gene sequencing of skates in Alaska. It confirms that egg case nurseries are essential to the genetic diversity and evolutionary success of these shark relatives. It also revealed something unexpected.
"We set out to use genetic analysis to find out how the two most abundant skate species in the Bering Sea are using nursery areas. How important is each nursery area? Are different nurseries used by distinct components of each species?" said Ingrid Spies, biologist at NOAA's Alaska Fisheries Science Center, who led the study. "We found answers to our questions, and a surprising result.
Did we accidentally discover a new species?"
Sharks and skates share an ancient lineage. Skates are distinguished by their flat body shape, suited for life near the seafloor. They are sometimes called "flat sharks".
Skates in Alaska: a vulnerable marine resource
Sixteen known skate species glide through Alaska seas. The Alaska skate makes up 90 percent of skate biomass on the Bering Sea shelf. The Aleutian skate predominates in deeper waters.
Skates are among the most vulnerable species in Alaska ecosystems. These large, long-lived fish grow slowly, mature late, and produce few offspring. All of this means skate populations are highly susceptible to the impacts of fishing and environmental change.
There is currently no targeted commercial skate fishery in Alaska. Nonetheless, skates are vulnerable to capture as they are caught unintentionally in other fisheries. Interest in developing a targeted skate fishery has grown as Alaskan communities seek to strengthen their economic resilience under changing environmental conditions.
Ensuring the sustainability of skate populations is a priority for NOAA Fisheries as part of an ecosystem-based approach to managing Alaska's valuable fisheries. To effectively manage skate populations and potentially develop a profitable fishery, it is essential to understand the significance of their egg case nurseries.
Skate nursery sites: What role do they play in preserving genetic diversity?
Skates produce their young in distinctive "mermaid purse" egg cases, familiar to beachcombers. The female skate is very particular about where she deposits her egg cases: the location must have the right slope, temperature, and oxygen level.
A skate nursery may support as many as 50,000 egg cases in a square kilometer area. Embryos develop there for as many as 5years before they hatch—one of the longest gestation periods of any vertebrate.
Scientists and fishery managers have recognized the importance of nursery sites as essential habitat for skates.
"It all started with Jerry Hoff's research on skate nursery areas in the Bering Sea," said Spies.
NOAA Fisheries biologist Hoff identified 26 skate egg nursery sites and proposed that eight of them be protected as important reproductive habitat. In 2013, the North Pacific Fishery Management Council designated six as Habitat Areas of Particular Concern.
But the reproductive behavior of skates associated with their nursery areas is still not fully understood.
"We have evidence that skates return to the same sites each year, similar to salmon returning to the streams where they hatched. If that is true, it has direct implications for conservation measures to protect habitat," Hoff explained. "We needed to understand how unique or isolated each skate egg nursery site may be, and to what degree each site contributes to the genetic diversity of the population."
Genetic detection of skate kinships
To begin to understand the relationship between Alaska skates and their nursery areas, the team turned to next-generation genetic sequencing methods.
"We asked: does skate embryo DNA differ among nursery areas?
Is it more different among sites than within?" Spies said.
Skate embryos were collected for genetic analysis from seven nursery areas in 2006, 2007, and 2016. Adult Alaska skates also were taken from several locations in the eastern Bering Sea and eastern Aleutian Islands.
Scientists extracted DNA from embryos and adults. They then compared single nucleotide polymorphisms, or SNPs (pronounced "snips") to determine how closely related skate embryos were within and among nurseries.
Findings: Nursery areas are uniquely important
Results of the study answered several of the researchers' questions, and raised some new ones.
- Skates return to the same nursery area each year
Genetic distinctiveness among nursery sites was consistent with skates depositing egg cases at the same nursery site each year.
"Distinct groups use specific nurseries," Spies said. "Nursery sites are really important for the diversity of skate species. If a nursery site disappeared, so would a distinct component of the species."
- More skate nursery areas probably exist that have not yet been discovered
The adult Alaska skates sampled were genetically distinct from the embryos.
"That suggests those adults came from unique nursery areas we haven't found yet," said Spies. "There is still more to their reproduction than we know about."
- Low effective population sizes underscore the importance of nursery sites
The study estimated effective population sizes for Alaska skates in one nursery. Effective population size is the number of individuals that effectively participates in producing the next generation. The smaller the effective population, the more it is at risk of losing genetic variation. Less genetic variation means less ability to adapt to environmental change, and higher risk of extinction.
"While higher than the threshold theoretically required for conservation of genetic diversity, the effective population size we observed for some of the nursery sites is still quite low," Spies said. "These results emphasize the need to protect skate egg nursery areas. Multiple genetically diverse nursery areas may help conserve genetic diversity in skate species if climate or other changes reduce the population size."
- Genetics may have revealed a cryptic new skate species
Genetic analysis of what appeared to be Alaska skate embryos yielded surprising results.
Some embryos, though visually indistinguishable from Alaska skates, were strikingly different genetically.
"Did we accidentally discover a new species? These embryos were as genetically different from Alaska skates as a separate species. As different as the leopard skate. There is no difference morphologically among embryos, but often embryos don't show differences. Maybe the adults look different as they grow. I would love to be involved in finding the adults, in describing a new species," said Spies. "If it is a new species, it will be important to be aware of its nursery area(s) to include it in our fishery management plan."
Nursery areas as an evolutionary strategy
"The results of this study are significant because they justify the importance of nursery areas as essential habitat to conserve genetic diversity in Alaskan skates," Spies said. "Our findings back up Hoff's idea that nursery sites should be protected as habitat areas of particular concern."
"The genetic component of our research demonstrates that the use of skate nurseries is not equal across the study area or the population. It reinforces the idea that skate reproduction is a complex system with many factors driving when, where, and how they use egg nursery sites," said Hoff.
"We've probably only begun to understand the complexity of skate reproduction," said Spies. "Think about nursery areas as an evolutionary strategy that supports the genetic diversity and evolutionary success of these species. Nursery areas are not only important for components of the population. Multiple nursery areas offer genetic diversity to the species to meet the ecological challenges they may face in a changing Alaska."